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2022.07.01

[Waters]시스테인을 단백질 샘플에서 분리하고 정량화하기 위한 샘플 준비 절차는 무엇입니까?

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•아미노산 분석
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답변

아미노산 시스테인 및 시스틴은 표준 단백질 가수 분해 프로토콜로 분해됩니다. 시스테인 모노머를 분석하고 단백질내 함량을 측정하기 위해, 수행 가능한 시료전처리 절차로 performic acid 산화(총 시스틴 및 시스테인 분석), carboxymethylation 또는 vinylpyridine과의 알킬화 및 환원 반응이 포함됩니다.

추가 정보

자세한 내용은 다음 저널 논문을 참조하십시오.

Fountoulakis M. and Lahm HW. "Hydrolysis and amino acid composition analysis of proteins." Journal of Chromatography A, 826(1998) 109-134.

또한 USP General Chapter 1052: Biotechnology derived articles for amino acid analysis도 참조하십시오.

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